PHYSIOCHEMICAL AND BIOLOGICAL PROPERTIES OF THE MAJOR BASIC PROTEIN FROM GUINEA PIG EOSINOPHIL GRANULES
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چکیده
منابع مشابه
Physiochemical and Biological Properties of the Major Basic Protein from Guinea Pig Eosinophil Granules
Guinea pig eosinophil granules are characterized by the presence of a basic protein of low molecular weight which accounts for greater than 50% of granule protein. This protein, termed the major basic protein (MBP), readily aggregates and becomes insoluble, and the formation of aggregates is dependent on the establishment of disulfide bonds. Analysis of concentrated preparations of MBP often re...
متن کاملIdentification of a Major Basic Protein in Guinea Pig Eosinophil Granules
Elucidation of the functions of the eosinophil might be accomplished by analysis of the granule constituents. We have purified eosinophils (93% or greater) from the peritoneal cavity of the guinea pig and have investigated a variety of methods to disrupt cells and liberate intact granules. Lysis in 0.34 M sucrose gave the best yield of granules and these had the characteristic morphology of eos...
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The localization of the guinea pig eosinophil major basic protein (MBP) within the cell was investigated by the use of immunoelectron microscopy and by isolation of the granule crystalloids. First, by immunoperoxidase electron microscopy, we found that the MBP of eosinophil granules is contained within the crystalloid core of the granule. Specific staining of cores was present when rabbit antis...
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Organ-specific highly basic protein derived from guinea pig central nervous system myelin was found to be electrophoretically heterogeneous. It did not appear to be heterogeneous with respect to molecular size. The electrophoretic technique used to demonstrate this heterogeneity was capable of resolving mixtures of closely related basic proteins whether they were present in crude extracts or in...
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The large specific granule of the human eosinophil contains a number of low molecular weight, highly basic proteins that have been purified to homogeneity, including the major basic protein (MBP) (1-3), 1 the eosinophil cationic protein (4, 5), and the eosinophil-derived neurotoxin (6, 7). The comparative physicochemical and immunochemical characteristics of these proteins have been examined in...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1974
ISSN: 1540-9538,0022-1007
DOI: 10.1084/jem.140.2.313